Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples-Reference-Cited by-同舟云学术

Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples

Published:2002-01 Issue:1 Volume:40 Page:287-291
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fang Ying¹,Wu Wai-Hong¹,Pepper Jessica L.¹,Larsen Jill L.¹,Marras Salvatore A. E.²,Nelson Eric. A.¹,Epperson William B.¹,Christopher-Hennings Jane¹

Affiliation:

1. Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007

2. Department of Molecular Genetics, Public Health Research Institute, New York, New York 10016

Abstract

ABSTRACT An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS 900 . A minimum quantity of 1.7 × 10 −4 pg of DNA, correlating to 1 to 8 CFU, was detected.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.1.287-291.2002

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