Imipenem-EDTA Disk Method for Differentiation of Metallo-β-Lactamase-Producing Clinical Isolates of Pseudomonas spp. and Acinetobacter spp

Author:

Yong Dongeun1,Lee Kyungwon12,Yum Jong Hwa2,Shin Hee Bong1,Rossolini Gian Maria3,Chong Yunsop1

Affiliation:

1. Department of Laboratory Medicine, Research Institute of Bacterial Resistance

2. Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea

3. Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita di Siena, I-53100 Siena, Italy

Abstract

ABSTRACT Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and −20°C, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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