Evaluation of a Strand Displacement Amplification Assay (BD ProbeTec-SDA) for Detection of Neisseria gonorrhoeae in Urine Specimens

Author:

Akduman Deniz1,Ehret Josephine M.12,Messina Kimberly1,Ragsdale Sharron1,Judson Franklyn N.123

Affiliation:

1. Denver Public Health Department

2. Departments of Medicine

3. Preventive Medicine, University of Colorado Health Sciences Center, Denver, Colorado

Abstract

ABSTRACT The performance of a strand displacement amplification assay (the BDProbeTec-SDA assay) in detecting Neisseria gonorrhoeae in urine specimens was evaluated. When performed under stringent quality control conditions, the BDProbeTec-SDA assay is a sensitive, specific, and efficient method for the screening of large numbers of noninvasively obtained specimens. Because the predictive value of an assay is a function of the prevalence of the disease, culture confirmation is needed for samples with positive results from populations in which the prevalence of a disease is low or in situations in which false-positive results may have important medical, psychosocial, or medicolegal consequences.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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4. Catry, M. A., M. J. Borrego, J. Cardoso, J. Azevedo, and I. Santo. 1995. Comparison of the Amplicor Chlamydia trachomatis test and cell culture for the detection of urogenital chlamydial infections. Genitourin. Med.71:247–250.

5. Chapin-Robertson, K. 1993. Use of molecular diagnostics in sexually transmitted diseases. Diagn. Microbiol. Infect. Dis.16:173–184.

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