Serological Differentiation of Infections with Dengue Virus Serotypes 1 to 4 by Using Recombinant Antigens-Reference-Cited by-同舟云学术

Serological Differentiation of Infections with Dengue Virus Serotypes 1 to 4 by Using Recombinant Antigens

Published:2002-11 Issue:11 Volume:40 Page:4317-4320
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ludolfs Diana¹,Schilling Stefan¹,Altenschmidt Jan¹,Schmitz Herbert¹

Affiliation:

1. Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany

Abstract

ABSTRACT The B domains of dengue virus serotypes 1 to 4 were expressed in Escherichia coli . The purified proteins were applied to immunoblot strips to detect serotype-specific antibodies in paired serum samples from 41 patients with primary and secondary dengue infections. A close correlation between the results obtained with the immunoblot strips and by type-specific reverse transcription-PCR (T. Laue, P. Emmerich, and H. Schmitz, J. Clin. Microbiol. 37: 2543-2547, 1999) was observed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.11.4317-4320.2002

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