Affiliation:
1. Tropical Disease Unit, Division of Infectious Diseases, Department of Medicine, University of Toronto, and Toronto General Hospital-University Health Network, Toronto, Canada
Abstract
ABSTRACT
Microscopy has been the traditional reference standard for malaria diagnosis. However, difficulty in maintaining the required technical skills, particularly in areas where malaria is not endemic, has prompted the development of rapid nonmicroscopic diagnostic assays based on the detection of malaria parasite antigen in whole blood. In this study, we evaluated the performance of one such device, the Makromed dipstick test, blindly compared to PCR and microscopy for the diagnosis of
Plasmodium falciparum
malaria in 200 febrile returned travelers. The Makromed assay detects the presence of
P. falciparum
-specific histidine-rich protein II with an antigen capture immunochromatographic strip format. Compared to PCR as the reference standard, the dipstick assay had a sensitivity of 97.0% and a specificity of 96.0%. The positive and negative predictive values were 81.2% and 99.5%, respectively. Rapid malaria diagnostic devices may provide a useful diagnostic adjunct in a clinical setting.
Publisher
American Society for Microbiology
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