Development of a Hexaplex PCR Assay for Rapid Detection of Virulence and Regulatory Genes in Vibrio cholerae and Vibrio mimicus-Reference-Cited by-同舟云学术

Development of a Hexaplex PCR Assay for Rapid Detection of Virulence and Regulatory Genes in Vibrio cholerae and Vibrio mimicus

Published:2002-11 Issue:11 Volume:40 Page:4321-4324
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Singh D. V.¹,Isac Sree Renjini¹,Colwell R. R.²³

Affiliation:

1. Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram 695 014, Kerala, India

2. Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202

3. Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742

Abstract

ABSTRACT We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A ( ctxA ), zonula occludens toxin ( zot ), accessory cholera enterotoxin ( ace ), toxin-coregulated pilus ( tcpA ), outer membrane protein ( ompU ), and central regulatory protein ToxR ( toxR ) in Vibrio cholerae and Vibrio mimicus . This hexaplex PCR proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic V. cholerae and V. mimicus strains from both clinical and environmental sources.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.11.4321-4324.2002

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