Affiliation:
1. National Laboratory for Enteric Pathogens, National Microbiology Laboratory, Winnipeg, Canada
Abstract
ABSTRACT
A multiplex PCR assay was used to simultaneously detect genes from the five major clinically relevant
Campylobacter
spp. Those genes selected were
hipO
and 23S rRNA from
Campylobacter jejuni
;
glyA
from each of
C. coli
,
C. lari
, and
C. upsaliensis
; and
sapB2
from
C. fetus
subsp.
fetus
. The assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures.
Publisher
American Society for Microbiology
Cited by
413 articles.
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