Phylogenetic Analysis of Newcastle Disease Virus Genotypes Isolated in Japan

Author:

Mase Masaji1,Imai Kunitoshi1,Sanada Yasuyuki2,Sanada Naoko3,Yuasa Noboru1,Imada Tadao1,Tsukamoto Kenji1,Yamaguchi Shigeo1

Affiliation:

1. National Institute of Animal Health, Tsukuba, Ibaraki 305-0856

2. Kyoritu Seiyaku Laboratories, Kukizaki-machi, Ibaraki 300-1252

3. Birds Hospital-BIRD HOUSE, Kashiwa, Chiba 277-0843, Japan

Abstract

ABSTRACT We genetically analyzed field isolates of the Newcastle disease (ND) virus isolated in Japan from 1930 to 2001. The coding region of the fusion protein was amplified by reverse transcriptase PCR and directly sequenced. Phylogenetic analysis revealed the presence of viruses belonging to six of the eight known genotypes. It can be concluded from this study that ND outbreaks in Japan have been of multiple etiologies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference44 articles.

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3. Ballagi-Pordany, A., E. Wehmann, J. Herczeg, S. Belak, and B. Lomniczi. 1996. Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene. Arch. Virol.141:243-261.

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