Simple and convenient method for culturing anaerobic bacteria

Author:

Behbehani M J,Jordan H V,Santoro D L

Abstract

A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic incubation of broth cultures in Brewer Anaerobic Jars.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference6 articles.

1. Preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria;Chan E. C. S.;J. Clin. Microbiol.,1978

2. Simple methods for culturing anaerobes. Appi;Davis C. E.;Microbiol.,1973

3. Hewitt L. F. 1950. Oxidation-reduction potentials in bacteriology and biochemistry 6th ed. E & S Livingstone Ltd. Edinburgh.

4. Holdeman L. V. E. P. Cato and W. E. C. Moore (ed). 1977. Anaerobic laboratory manual 4th ed. Virginia Polytechnic Institute and State University Blacksburg Va.

5. Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections;Moore W. E. C.;J. Infect. Dis.,1969

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