Affiliation:
1. Biology Group, Research Council of Alberta, Edmonton, Alberta, Canada T6G 2C2
Abstract
An anaerobic mesophilic digestor was operated using beef cattle waste (diluted to 5.75% volatile solids) as substrate; retention time was 10 days with daily batch feed. Volatile solids destruction was 36%. Daily gas production rate was 1.8 liters of gas (standard temperature and pressure) per liter of digestor contents (0.99 liters of CH
4
per liter of digestor contents). Acetate turnover was measured, and it was calculated that 68% of the CH
4
was derived from the methyl group of acetate. When the methanogenic substrates acetic acid or H
2
/CO
2
were added to the digestor on a continuous basis, the microflora were able to adapt and convert them to terminal products while continuing to degrade animal waste to the same extent as without additions. The methanogenic substrates were added at a rate at least 1.5 times the microbial production rate which was measured in the absence of added substrates. Added acetate was converted directly to CH
4
by acetoclastic methanogens; H
2
addition greatly stimulated acetate production in the digestor. A method is described for the measurement of acetate turnover in batch-fed digestors.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference21 articles.
1. Association of Official Agricultural Chemists. 1965. Official methods of analysis 10th ed. Association of Official Agricultural Chemists Washington D.C.
2. Methanogenesis from acetate: enrichment studies;Baresi L.;Appl. Environ. Microbiol.,1978
3. Propionate-degrading bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from methanogenic ecosystems;Boone D. R.;Appl. Environ. Microbiol.,1980
4. Microbial methane production-theoretical aspects;Bryant M. P.;J. Anim. Sci.,1979
5. Substrate utilization kinetic model for biological treatment processes;Chen Y. R.;Biotechnol. Bioeng.,1980
Cited by
95 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献