A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms

Author:

Wang San-Pin1,Kuo Cho-Chou1,Grayston J. Thomas1

Affiliation:

1. Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195

Abstract

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference11 articles.

1. Further classification of TRIC strains from ocular trachoma and other sources by the mouse toxicity prevention test;Alexander E. R.;Amer. J. Ophthalmol.,1967

2. The titration of trachoma and inclusion blennorrhoea viruses in cell cultures;Furness G.;J. Gen. Microbiol.,1960

3. Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation;Gordon F. B.;J. Infect. Dis.,1969

4. Kuo C. C. G. E. Kenny and S. P. Wang. 1971. Trachoma and psittacosis antigens in agar gel double immunodiffusion p. 113-123. In R. L. Nichols (ed.) Trachoma (Proceedings of the Symposium held in Boston Massachusetts August 1970). Excerpta Medica Amsterdam.

5. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAEdextran;Kuo C. C.;J. Infect. Dis.,1972

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3