Affiliation:
1. Department of Medicine, State University of New York at Buffalo, Buffalo, New York 14215
Abstract
The β antigen of
Neisseria gonorrhoeae
has been isolated from the alkaline-extracted gonococcal endotoxin by ion exchange, molecular sieve, and powder block electrophoretic chromatography. Hemagglutination inhibition studies indicate that the preparation is essentially free of common enterobacterial and gonococcal α antigen. Analysis of the isolated antigen by immunodiffusion and acrylamide gel electrophoresis reveal only one detectable component. Chemical studies indicate that the antigen is an acidic glycoprotein composed primarily of four major amino acids: alanine, glutamic acid, glycine, and proline. The antigen has an
s
0,w
of 8.55, and spectral analysis in the ultraviolet and visible range reveals a single absorption peak at 217 nm.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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