Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model-Reference-Cited by-同舟云学术

Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model

Published:1997-08 Issue:8 Volume:65 Page:3474-3477
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Bénitez J A¹,Spelbrink R G¹,Silva A¹,Phillips T E¹,Stanley C M¹,Boesman-Finkelstein M¹,Finkelstein R A¹

Affiliation:

1. Centro Nacional de Investigaciones Científicas, Havana, Cuba.

Abstract

Choleragenic vibrios adhered to and multiplied on monolayers of the highly differentiated mucin-secreting cell line HT29-18N2. Their adherence followed first-order kinetics, was dependent on the concentration of vibrios, and was partially inhibited by lipopolysaccharide. Comparison of genetically modified vibrios showed that flagella, an active toxR gene, and the virulence cassette were not essential for initial binding. Inactivation of the hemagglutinin/protease increased binding. This highly differentiated human intestinal cell line provides a versatile new approach for studying major events occurring during intestinal colonization: adherence, multiplication, and detachment.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.65.8.3474-3477.1997

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