Affiliation:
1. Centre “Bioengineering,” Russian Academy of Sciences, Moscow, 117312, Russia
Abstract
ABSTRACT
The physiological conditions and molecular interactions that control phage production have been studied in only a few families of temperate phages. We investigated the mechanisms that regulate activation of lytic development in lysogens of coliphage N15, a prophage that is not integrated into the host chromosome but exists as a linear plasmid with covalently closed ends. We identified the N15 antirepressor gene,
antC
, and showed that its product binds to and acts against the main phage repressor, CB. LexA binds to and represses the promoter of
antC
. Mitomycin C-stimulated N15 induction required RecA-dependent autocleavage of LexA and expression of AntC protein. Thus, a cellular repressor whose activity is regulated by DNA damage controls N15 prophage induction.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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