Shortcut Method for Extraction of Staphylococcus aureus DNA from Blood Cultures and Conventional Cultures for Use in Real-Time PCR Assays-Reference-Cited by-同舟云学术

Shortcut Method for Extraction of Staphylococcus aureus DNA from Blood Cultures and Conventional Cultures for Use in Real-Time PCR Assays

Published:2005-06 Issue:6 Volume:43 Page:2932-2933
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Adams D. Nicholas¹

Affiliation:

1. Department of Microbiology, Royal Prince Alfred Hospital, Missenden Rd., Camperdown 2050, NSW, Australia

Abstract

ABSTRACT PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.6.2932-2933.2005

Reference15 articles.

1. Identification and Characterization of Immunoglobulin G in Blood as a Major Inhibitor of Diagnostic PCR

2. Purification and Characterization of PCR-Inhibitory Components in Blood Cells

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4. Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay

5. Improved Amplification of Microbial DNA from Blood Cultures by Removal of the PCR Inhibitor Sodium Polyanetholesulfonate

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