Affiliation:
1. School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia 5005, Australia
Abstract
ABSTRACT
We have recently described a novel AB
5
subtilase cytotoxin produced by certain Shiga toxigenic
Escherichia coli
(STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene
subA
, as well as
stx
1
and
stx
2
. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.
Publisher
American Society for Microbiology
Cited by
93 articles.
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