Protective Efficacy of the Conserved NP, PB1, and M1 Proteins as Immunogens in DNA- and Vaccinia Virus-Based Universal Influenza A Virus Vaccines in Mice

Author:

Wang Wenling1,Li Renqing12,Deng Yao1,Lu Ning1,Chen Hong1,Meng Xin1,Wang Wen1,Wang Xiuping1,Yan Kexia1,Qi Xiangrong1,Zhang Xiangmin1,Xin Wei1,Lu Zhenhua1,Li Xueren1,Bian Tao1,Gao Yingying1,Tan Wenjie1,Ruan Li1

Affiliation:

1. National Institute for Viral Disease Control & Prevention, Chinese Center for Disease Control and Prevention (China CDC), Chang Ping District, Beijing, China

2. Institute of Immunization and Prevention, Beijing Center for Disease Control and Prevention, Dong Cheng District, Beijing, China

Abstract

ABSTRACT The conventional hemagglutinin (HA)- and neuraminidase (NA)-based influenza vaccines need to be updated most years and are ineffective if the glycoprotein HA of the vaccine strains is a mismatch with that of the epidemic strain. Universal vaccines targeting conserved viral components might provide cross-protection and thus complement and improve conventional vaccines. In this study, we generated DNA plasmids and recombinant vaccinia viruses expressing the conserved proteins nucleoprotein (NP), polymerase basic 1 (PB1), and matrix 1 (M1) from influenza virus strain A/Beijing/30/95 (H3N2). BALB/c mice were immunized intramuscularly with a single vaccine based on NP, PB1, or M1 alone or a combination vaccine based on all three antigens and were then challenged with lethal doses of the heterologous influenza virus strain A/PR/8/34 (H1N1). Vaccines based on NP, PB1, and M1 provided complete or partial protection against challenge with 1.7 50% lethal dose (LD 50 ) of PR8 in mice. Of the three antigens, NP-based vaccines induced protection against 5 LD 50 and 10 LD 50 and thus exhibited the greatest protective effect. Universal influenza vaccines based on the combination of NP, PB1, and M1 induced a strong immune response and thus might be an alternative approach to addressing future influenza virus pandemics.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

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