Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus

Author:

Ligozzi M1,Rossolini G M1,Tonin E A1,Fontana R1

Affiliation:

1. Institute of Microbiology, University of Verona, Italy.

Abstract

A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference24 articles.

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3. Intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus;Brown F. J.;FEBS Lett.,1980

4. Methicillin-resistant staphylococci;Chambers H. F.;Clin. Microbiol. Rev.,1988

5. Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus;Froggatt J. W.;Antimicrob. Agents Chemother.,1989

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