The Amino-Terminal Region of Major Capsid Protein P3 Is Essential for Self-Assembly of Single-Shelled Core-Like Particles of Rice Dwarf Virus-Reference-Cited by-同舟云学术

The Amino-Terminal Region of Major Capsid Protein P3 Is Essential for Self-Assembly of Single-Shelled Core-Like Particles of Rice Dwarf Virus

Published:2004-03-15 Issue:6 Volume:78 Page:3145-3148
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Hagiwara Kyoji¹,Higashi Takahiko¹,Miyazaki Naoyuki²,Naitow Hisashi³,Cheng R. Holland⁴,Nakagawa Atsushi²,Mizuno Hiroshi⁵,Tsukihara Tomitake²,Omura Toshihiro¹

Affiliation:

1. Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666

2. Institute for Protein Research, Osaka University, Suita, Osaka 565-0871

3. Division of Bio-Crystallography Technology, RIKEN Harima Institute, Sayo-gun, Hyogo 679-5148

4. Department of Biosciences at Novum, Karolinska Institute, 14157 Huddinge, Sweden

5. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan

Abstract

ABSTRACT The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one of which is inserted by the amino-terminal region of another P3 protein. The P3 proteins with serial amino-terminal deletions, expressed in a baculovirus system, formed particles with gradually decreasing stability. The capacity for self-assembly disappeared when 52 of the amino-terminal amino acids had been deleted. These results demonstrated that insertion of the amino-terminal arm of one P3 protein into another appears to play an important role in stabilizing the core particles.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.78.6.3145-3148.2004

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