Affiliation:
1. Department of Cellular Biology, Genetics and Animal Physiology, Málaga University, Málaga 29071, Spain
2. Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695
Abstract
ABSTRACT
Geminiviruses are small DNA viruses that replicate in nuclei of infected plant cells after accumulation of host replication machinery.
Tomato golden mosaic virus
(TGMV) and
Tomato yellow leaf curl Sardinia virus
(TYLCSV) encode a protein, RepAC1 (or Rep), that is essential for viral replication. Rep/RepAC1 is an oligomeric protein that binds to double-stranded DNA, catalyzes cleavage and ligation of single-stranded DNA, and is sufficient for host induction. It also interacts with several host proteins, including the cell cycle regulator, retinoblastoma, and essential components of the cell DNA replication machinery, like proliferating nuclear cell antigen (PCNA) and RFC-1. To identify other cellular proteins that interact with Rep/RepAC1 protein, a
Nicotiana benthamiana
cDNA library was screened with a yeast two-hybrid assay. The host cell sumoylation enzyme, NbSCE1 (
N. benthamiana
SUMO-conjugating enzyme, homolog to
Saccharomyces cerevisiae
UBC9), was found to interact specifically with RepAC1. Mapping studies localized the interaction to the N-terminal half of RepAC1. Effects on geminivirus replication were observed in transgenic plants with altered levels of SUMO, the substrate for UBC9.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
104 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献