Affiliation:
1. Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachussetts 02454-9110
Abstract
ABSTRACT
Repair of double-strand breaks by gene conversions between homologous sequences located on different
Saccharomyces cerevisiae
chromosomes or plasmids requires
RAD51
. When repair occurs between inverted repeats of the same plasmid, both
RAD51
-dependent and
RAD51
-independent repairs are found. Completion of
RAD51
-independent plasmid repair events requires
RAD52
,
RAD50
,
RAD59
,
TID1
(
RDH54
), and
SRS2
and appears to involve break-induced replication coupled to single-strand annealing. Surprisingly,
RAD51
-independent recombination requires much less homology (30 bp) for strand invasion than does
RAD51
-dependent repair (approximately 100 bp); in fact, the presence of Rad51p impairs recombination with short homology. The differences between the
RAD51
- and
RAD50
/
RAD59
-dependent pathways account for the distinct ways that two different recombination processes maintain yeast telomeres in the absence of telomerase.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
162 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献