Affiliation:
1. Departments of Genetics and Development and Microbiology
2. Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University, New York, New York 10032
Abstract
ABSTRACT
The Snf1 protein kinase of
Saccharomyces cerevisiae
is important for many cellular responses to glucose limitation, including haploid invasive growth. We show here that Snf1 regulates transcription of
FLO11
, which encodes a cell surface glycoprotein required for invasive growth. We further show that Nrg1 and Nrg2, two repressor proteins that interact with Snf1, function as negative regulators of invasive growth and as repressors of
FLO11
. We also examined the role of Snf1, Nrg1, and Nrg2 in two other Flo11-dependent processes. Mutations affected the initiation of biofilm formation, which is glucose sensitive, but also affected diploid pseudohyphal differentiation, thereby unexpectedly implicating Snf1 in a response to nitrogen limitation. Deletion of the
NRG1
and
NRG2
genes suppressed the defects of a
snf1
mutant in all of these processes. These findings suggest a model in which the Snf1 kinase positively regulates Flo11-dependent developmental events by antagonizing Nrg-mediated repression of the
FLO11
gene.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
181 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献