Application of charge shift electrophoresis to antigenic analysis of mycoplasmic membranes by two-dimensional (crossed) immunoelectrophoresis-Reference-Cited by-同舟云学术

Application of charge shift electrophoresis to antigenic analysis of mycoplasmic membranes by two-dimensional (crossed) immunoelectrophoresis

Published:1978-06 Issue:3 Volume:20 Page:861-863
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Alexander A G,Kenny G E

Abstract

Both the anodic migration and resolution of membrane antigens of Mycoplasma arginini were greatly improved in two-dimensional immunoelectrophoresis by incorporating both a neutral detergent, Triton X-100, and an anionic detergent, sodium deoxycholate, in the agarose support for first phase electrophoresis. The mobility of cytoplasmic antigens was unaffected at concentrations of 0.1% or less of sodium deoxycholate.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.20.3.861-863.1978

Reference10 articles.

1. Characterization of membrane and cytoplasmic antigens of Mycoplasma arginini by two-dimensional (crossed) immunoelectrophoresis;Alexander A. G.;Infect. Immun.,1977

2. Detection of amphiphilic proteins and peptides in complex mixtures: charge-shift crossed immunoelectrophoresis and two-dimensional charge-shift electrophoresis;Bhakdi S.;Biochim. Biophys. Acta,1977

3. Antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. I. Antigenic heterogeneity between Chlamydia trachomatis and Chlamydiapsittaci;Caldwell H. D.;J. Immunol.,1975

4. Charge shift electrophoresis: simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution;Helenius A.;Proc. Natl. Acad. Sci. U.S.A.,1977

5. Localization of the Tween 20 soluble membrane proteins of Acholeplasma laidlawii by crossed immunoelectrophoresis;Johansson K.;J. Mol. Biol.,1974

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