Clostridioides difficile Whole-Genome Sequencing Reveals Limited Within-Host Genetic Diversity in a Pediatric Cohort-Reference-Cited by-同舟云学术

Clostridioides difficile Whole-Genome Sequencing Reveals Limited Within-Host Genetic Diversity in a Pediatric Cohort

Published:2019-09 Issue:9 Volume:57 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Balaji Aakash¹,Ozer Egon A.²^ORCID,Kociolek Larry K.¹³

Affiliation:

1. Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA

2. Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA

3. Division of Pediatric Infectious Diseases, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois, USA

Abstract

Whole-genome sequencing (WGS) is a highly sensitive method for identifying genetic relatedness and transmission of Clostridioides difficile strains. Previous studies suggest that as few as 3 core genome single-nucleotide variants (SNVs) discriminate between genetically distinct isolates. Because a single C. difficile colony is selected from culture for WGS, significant within-host genetic diversity could preclude identification of transmission events.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

American Cancer Society

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00559-19

Reference15 articles.

1. Burden ofClostridium difficileInfection in the United States

2. Diverse Sources of C. difficile Infection Identified on Whole-Genome Sequencing