Affiliation:
1. Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center
2. The OSU RNA Group, Ohio State University, Columbus, Ohio 43210
Abstract
ABSTRACT
RNA motifs comprising nucleotides that interact through non-Watson-Crick base pairing play critical roles in RNA functions, often by serving as the sites for RNA-RNA, RNA-protein, or RNA small ligand interactions. The structures of viral and viroid RNA motifs are studied commonly by in vitro, computational, and mutagenesis approaches. Demonstration of the in vivo existence of a motif will help establish its biological significance and promote mechanistic studies on its functions. By using UV cross-linking and primer extension, we have obtained direct evidence for the in vivo existence of the loop E motif of
Potato spindle tuber viroid
. We present our findings and discuss their biological implications.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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