Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR-Reference-Cited by-同舟云学术

Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR

Published:1996-04 Issue:4 Volume:62 Page:1478-1480
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Briglia M¹,Eggen R I¹,de Vos W M¹,van Elsas J D¹

Affiliation:

1. Institute for Plant Protection, Wageningen, Netherlands.

Abstract

A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.62.4.1478-1480.1996

Reference17 articles.

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3. Phylogenetic evidence for the transfer of the pentachlorophenol-mineralizing Rhodococcus chlorophenolicus strain PCP-1 to the genus;Briglia M.;Mycobacterium. Int. J. Syst. Bacteriol.,1994

4. Mineralization performance of Rhodococcus chlorophenolicus strain PCP-1 in contaminated soil simulating on site conditions;Briglia M.;Soil Biol. Biochem.,1994

5. The survival of the pentachlorophenol-mineralizing Rhodococcus chlorophenolicus PCP-1 and Flavobacterium sp. in natural soil;Briglia M.;Biodegradation,1990

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