Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli-Reference-Cited by-同舟云学术

Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli

Published:1996-05 Issue:5 Volume:62 Page:1808-1810
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Millard C S¹,Chao Y P¹,Liao J C¹,Donnelly M I¹

Affiliation:

1. Department of Chemical Engineering, Texas A&M University, College Station 77843, USA.

Abstract

Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold increase in the concentration of succinic acid, making succinic acid the major fermentation product by weight.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.62.5.1808-1810.1996

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