Involvement of Vitamin B 6 in the Dethiomethylation of Methionine by Rumen Microorganisms

Author:

Merricks D. L.1,Salsbury R. L.1

Affiliation:

1. Department of Animal Science and Agricultural Biochemistry, University of De'aware, Newark, Delaware 19711

Abstract

The dethiomethylation of methionine by a dialyzed extract obtained from the protozoa-rich fraction of rumen fluid is stimulated 2.5-fold by pyridoxal phosphate and strongly inhibited by deoxypyridoxine, a pyridoxal phosphate antagonist. These effects are not seen with undialyzed extracts or with whole rumen fluid. It is suggested that the anaerobic dethiomethylation of methionine by rumen microorganisms requires pyridoxal phosphate as a cofactor.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference15 articles.

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2. Pyridoxal phosphate. Annu;Fasella P.;Rev. Biochem.,1967

3. Decomposition of thioester derivatives by bacteria. I. Methylmercaptan formation and the properties of the responsible enzyme;Mitsuhashi S.;Jap. J. Exp. Biol.,1949

4. Decomposition of thioester derivatives by bacteria. II. a-Keto acid as a product in the enzymatic cleavage of thioester derivatives;Mitsuhashi S.;Jap. J. Exp. Biol.,1950

5. Enzymatic cleavage of methionine and homocysteine by bacteria;Miwatani T.;Med. J. Osaka Univ.,1954

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