Large-Scale, In-House Production of Viral Transport Media To Support SARS-CoV-2 PCR Testing in a Multihospital Health Care Network during the COVID-19 Pandemic

Author:

Smith Kenneth P.12,Cheng Annie1,Chopelas Amber1,DuBois-Coyne Sarah13,Mezghani Ikram4,Rodriguez Shade1,Talay Mustafa5,Kirby James E.12

Affiliation:

1. Department of Pathology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA

2. Harvard Medical School, Boston, Massachusetts, USA

3. Department of Biochemistry, University of Massachusetts Boston, Boston, Massachusetts, USA

4. Department of Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA

5. Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA

Abstract

The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCR) testing. In response to this ongoing shortage, we began production of VTM in-house in support of diagnostic testing in our hospital network. As our diagnostic laboratory was not equipped for reagent production, we took advantage of space and personnel that became available due to closure of the research division of our medical center.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference7 articles.

1. Open Development and Clinical Validation Of Multiple 3D-Printed Sample-Collection Swabs: Rapid Resolution of a Critical COVID-19 Testing Bottleneck

2. Centers for Disease Control and Prevention. 2020. DSR-052-02: preparation of viral transport medium. https://www.cdc.gov/coronavirus/2019-ncov/downloads/Viral-Transport-Medium.pdf. Accessed 27 April 2020.

3. How to optimize the drop plate method for enumerating bacteria

4. Predicting the Stability of Biological Standards and Products

5. Clinical and Laboratory Standards Institute. 2009. Evaluation of stability of in vitro diagnostic reagents. CLSI document EP25-A. Clinical and Laboratory Standards Institute, Wayne, PA.

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