Characterization of the Streptococcus mutans Pyruvate Formate-Lyase (PFL)-Activating Enzyme Gene by Complementary Reconstitution of the In Vitro PFL-Reactivating System-Reference-Cited by-同舟云学术

Characterization of the Streptococcus mutans Pyruvate Formate-Lyase (PFL)-Activating Enzyme Gene by Complementary Reconstitution of the In Vitro PFL-Reactivating System

Published:2000-08 Issue:8 Volume:68 Page:4773-4777
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Yamamoto Yasuhito¹,Sato Yutaka¹²,Takahashi-Abbe Shoko³,Takahashi Nobuhiro³,Kizaki Harutoshi¹²

Affiliation:

1. Department of Biochemistry1 and

2. Oral Health Science Center,2 Tokyo Dental College, Mihama-ku, Chiba City 261-8502, and

3. Department of Oral Biochemistry, School of Dentistry, Tohoku University, Aoba-ku, Sendai City 980-8575,3 Japan

Abstract

ABSTRACT The act gene was identified and an act mutant as well as the pfl mutant was constructed in Streptococcus mutans . Pyruvate formate-lyase (PFL) activity was regenerated with the mixture of the respective cell extracts from these mutants by complementary reconstitution of the in vitro reactivating system. The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.68.8.4773-4777.2000

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