Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from Actinoplanes utahensis-Reference-Cited by-同舟云学术

Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from Actinoplanes utahensis

Published:2007-08-15 Issue:16 Volume:73 Page:5378-5381
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Torres-Bacete Jesús¹,Hormigo Daniel¹,Stuart Maribel¹,Arroyo Miguel¹,Torres Pedro¹,Castillón María P.¹,Acebal Carmen¹,García José L.²,de la Mata Isabel¹

Affiliation:

1. Departamento de Bioquímica y Biología Molecular I, Facultad de Biología, Universidad Complutense, C/ José Antonio Nováis 2, 28040 Madrid, Spain

2. Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, CSIC, C/ Ramiro de Maeztu 9, 28040 Madrid, Spain

Abstract

ABSTRACT Aculeacin A acylase from Actinoplanes utahensis produced by Streptomyces lividans revealed acylase activities that are able to hydrolyze penicillin V and several natural aliphatic penicillins. Penicillin K was the best substrate, showing a catalytic efficiency of 34.79 mM −1 s −1 . Furthermore, aculeacin A acylase was highly thermostable, with a midpoint transition temperature of 81.5°C.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00452-07

Reference21 articles.

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