Genetic Manipulation of Wild Human Gut Bacteroides

Author:

Bencivenga-Barry Natasha A.12,Lim Bentley12,Herrera Carmen M.34,Trent M. Stephen345,Goodman Andrew L.12ORCID

Affiliation:

1. Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, USA

2. Microbial Sciences Institute, Yale University, West Haven, Connecticut, USA

3. Department of Infectious Diseases, University of Georgia at Athens, College of Veterinary Medicine, Athens, Georgia, USA

4. Center for Vaccines and Immunology, University of Georgia at Athens, College of Veterinary Medicine, Athens, Georgia, USA

5. Department of Microbiology, University of Georgia at Athens, College of Arts and Sciences, Athens, Georgia, USA

Abstract

Human gut Bacteroides species exhibit strain-level differences in their physiology, ecology, and impact on human health and disease. However, existing approaches for genetic manipulation generally require construction of genetically modified parental strains for each microbe of interest or defined medium formulations. In this report, we introduce a robust and efficient strategy for targeted genetic manipulation of diverse wild-type Bacteroides species from the human gut. This system enables genetic investigation of members of human and animal microbiomes beyond existing model organisms.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

HHS | NIH | National Institute of General Medical Sciences

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

Howard Hughes Medical Institute

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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