Immunological Fingerprinting Method for Differentiation of Serum Samples in Research-Oriented Biobanks

Author:

Beaumont Katy1,Betsou Fotini1

Affiliation:

1. Biobanque de Picardie, Centre Hospitalier Universitaire d'Amiens, av. René Laënnec, 80480 Salouel, France

Abstract

ABSTRACT An immunoenzymatic serum fingerprinting method was developed to establish a serum sample fingerprint based on IgG titers obtained with three different antigens. Three widely expressed antigens were selected for their capacity to induce long-lasting humoral immune responses. This fingerprinting method may be used to differentiate between two serum samples and to determine whether they come from the same primary blood specimen. The method showed a specificity of 99.5%. This method is suitable as a quality control method for biobanked serum samples.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference24 articles.

1. Ascherio, A., K. L. Munger, E. T. Lennette, D. Spiegelman, M. A. Herman, J. Olek, S. E. Hankinson, and D. J. Hunter. 2001. Epstein-Barr virus antibodies and risk of multiple sclerosis: a prospective study. JAMA26:3083-3088.

2. Betsou, F. 2006. Biobanks in immunoanalysis and specialized biology. Immunoanal. Biol. Spec.21:327-332.

3. Blake, N., T. Haigh, G. Shaka'a, D. Croom-Carter, and A. Rickinson. 2000. The importance of exogenous antigen in priming the human CD8+ T cell response: lessons from EBV nuclear antigen EBNA-1. J. Immunol.165:7078-7087.

4. Chaigneau, C., T. Cabioch, K. Beaumont, and F. Betsou. 2007. Serum biobank certification and the establishment of quality controls for biological fluids: examples of serum biomarker stability after temperature variation. Clin. Chem. Lab Med.45:1390-1395.

5. Prevalence of Specific Antibodies to Chlamydia pneumoniae in Korea

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