Affiliation:
1. Department of Bacteriology, University of California, Davis, California 95616
Abstract
The episomic element F′
lac
+
was transferred, probably by conjugation, from
Escherichia coli
to Lac
−
strains of
Erwinia herbicola, Erwinia amylovora
, and
Erwinia chrysanthemi
(but not to several other
Erwinia
spp. In preliminary trials). The
lac
genes in the exconjugants of the
Erwinia
spp. showed varying degrees of stability depending on the strain (stable in
E. herbicola
strains Y46 and Y74 and
E. amylovora
strain EA178, but markedly unstable in
E. chrysanthemi
strain EC16). The
lac
genes and the sex factor (F) were eliminated from the exconjugants by treatment with acridine orange, thus suggesting that both
lac
and F are not integrated in the
Erwinia
exconjugants. All of the tested Lac
+
exconjugants of
E. herbicola
strains Y46 and Y74 and
E. amylovora
strain EA178, but not of
E. chrysanthemi
strain EC 16, were sensitive to the F-specific phage M13. The heterogenotes (which harbored F′
lac
+
) of
E. herbicola
strains Y46 and Y74,
E. amylovora
strain EA178, and
E. chrysanthemi
strain EC16 were able to transfer
lac
genes by conjugation to strains of
E. herbicola, E. amylovora, E. chrysanthemi, Escherichia coli
, and
Shigella dysenteriae
. The frequency of such transfer from Lac
+
exconjugants of
Erwinia
spp. was comparable to that achieved by using
E. coli
F′
lac
+
as donors, thus indicating the stability, expression, and restriction-and-modification properties of the sex factor (F) in
Erwinia
spp.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
39 articles.
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