Practical Method for Extraction of PCR-Quality DNA from Environmental Soil Samples-Reference-Cited by-同舟云学术

Practical Method for Extraction of PCR-Quality DNA from Environmental Soil Samples

Published:2010-07 Issue:13 Volume:76 Page:4571-4573
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Fitzpatrick Kelly A.¹,Kersh Gilbert J.¹,Massung Robert F.¹

Affiliation:

1. Rickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Abstract

ABSTRACT Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02825-09

Reference8 articles.

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