Pyrosequencing Analysis of 20 Nucleotides of Internal Transcribed Spacer 2 Discriminates Candida parapsilosis , Candida metapsilosis , and Candida orthopsilosis-Reference-Cited by-同舟云学术

Pyrosequencing Analysis of 20 Nucleotides of Internal Transcribed Spacer 2 Discriminates Candida parapsilosis , Candida metapsilosis , and Candida orthopsilosis

Published:2009-07 Issue:7 Volume:47 Page:2307-2310
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Borman Andrew M.¹,Linton Christopher J.¹,Oliver Debra¹,Palmer Michael D.¹,Szekely Adrien¹,Odds Frank C.²,Johnson Elizabeth M.¹

Affiliation:

1. Mycology Reference Laboratory, Health Protection Agency, Bristol, United Kingdom

2. Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

Abstract

ABSTRACT Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis , were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00240-09

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