Novel Application of the Masson-Fontana Stain for Demonstrating Malassezia Species Melanin-Like Pigment Production In Vitro and in Clinical Specimens

Author:

Gaitanis George1,Chasapi Vassiliki2,Velegraki Aristea1

Affiliation:

1. Mycology Reference Laboratory (Hellenic Centre for Diseases Control), Microbiology Department, Medical School, University of Athens

2. Third Dermatology Department, National Health System, “A. Sygros” Hospital, Athens, Greece

Abstract

ABSTRACT Melanin-like pigment produced in vitro and in vivo by Malassezia yeasts has not been described before. Masson-Fontana staining confirmed accumulation of black pigment on the cell walls of l -dihydroxyphenylalaline ( l -DOPA)-cultured Malassezia species. Black pigment was also observed in cells and hyphae from hyperpigmented patient lesions with culture-confirmed pityriasis versicolor and seborrheic dermatitis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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