Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates

Author:

Diancourt Laure1,Passet Virginie1,Verhoef Jan2,Grimont Patrick A. D.1,Brisse Sylvain1

Affiliation:

1. Unité Biodiversité des Bactéries Pathogènes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France

2. Eijkman-Winkler Center, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands

Abstract

ABSTRACT A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae . Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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