Affiliation:
1. Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark
Abstract
ABSTRACT
Genes involved in allose utilization of
Escherichia coli
K-12 are organized in at least two operons,
alsRBACE
and
alsI
, located next to each other on the chromosome but divergently transcribed. Mutants defective in
alsI
(allose 6-phosphate isomerase gene) and
alsE
(allulose 6-phosphate epimerase gene) were Als
−
. Transcription of the two allose operons, measured as β-galactosidase activity specified by
alsI-lacZ
+
or
alsE-lacZ
+
operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
42 articles.
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