Occurrence of Free d -Amino Acids and Aspartate Racemases in Hyperthermophilic Archaea-Reference-Cited by-同舟云学术

Occurrence of Free d -Amino Acids and Aspartate Racemases in Hyperthermophilic Archaea

Published:1999-10-15 Issue:20 Volume:181 Page:6560-6563
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Matsumoto Megumi¹,Homma Hiroshi¹,Long Zhiqun¹,Imai Kazuhiro¹,Iida Toshii²,Maruyama Tadashi²,Aikawa Yuko³,Endo Isao³,Yohda Masafumi⁴

Affiliation:

1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033,1

2. Marine Biotechnology Institute, Kamaishi, Iwate 021-0001,2 and

3. Biochemical Systems Laboratory, The Institute of Physical and Chemical Research, Wako, Saitama 351-0198,3 Japan

4. Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588,4

Abstract

ABSTRACT The occurrence of free d -amino acids and aspartate racemases in several hyperthermophilic archaea was investigated. Aspartic acid in all the hyperthermophilic archaea was highly racemized. The ratio of d -aspartic acid to total aspartic acid was in the range of 43.0 to 49.1%. The crude extracts of the hyperthermophiles exhibited aspartate racemase activity at 70°C, and aspartate racemase homologous genes in them were identified by PCR. d -Enantiomers of other amino acids (alanine, leucine, phenylalanine, and lysine) in Thermococcus strains were also detected. Some of them might be by-products of aspartate racemase. It is proven that d -amino acids are produced in some hyperthermophilic archaea, although their function is unknown.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.181.20.6560-6563.1999

Reference28 articles.

1. Bada J. L. Racemization of amino acids Chemistry and biochemistry of amino acids. Barrett G. C. 1985 399 414 Chapman and Hall New York N.Y

2. Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii

3. The presence of free d-aspartic acid in rodents and man;Dunlop D. S.;Biochem. Biophys. Res. Commun.,1986

4. Rapid racemization of aspartic acid in mollusc shells and potential for dating over recent centuries;Goodfriend G. A.;Nature,1992

5. A comparative study of the kinetics of amino acid racemization/epimerization in fossil and modern mollusk shells;Goodfriend G. A.;Geochim. Cosmochim. Acta,1991

Cited by 43 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Novel D-glutamate catabolic pathway in marine Proteobacteria and halophilic archaea;The ISME Journal;2023-01-23

2. Aspartic acid racemization and repair in the survival and recovery of hyperthermophiles after prolonged starvation at high temperature;FEMS Microbiology Ecology;2021-07-29

3. Characterization of a novel moderate-substrate specificity amino acid racemase from the hyperthermophilic archaeon Thermococcus litoralis;Bioscience, Biotechnology, and Biochemistry;2021-05-04

4. Effect of chirality of cysteine on the cell growth and photo-fermentative hydrogen production by using acetate as substrate;Fuel;2021-04

5. TK1211 Encodes an Amino Acid Racemase towards Leucine and Methionine in the Hyperthermophilic Archaeon Thermococcus kodakarensis;Journal of Bacteriology;2021-03-08