Isolation and Characterization of the nikR Gene Encoding a Nickel-Responsive Regulator in Escherichia coli-Reference-Cited by-同舟云学术

Isolation and Characterization of the nikR Gene Encoding a Nickel-Responsive Regulator in Escherichia coli

Published:1999-01-15 Issue:2 Volume:181 Page:670-674
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

De Pina Karinne¹,Desjardin Valerie¹,Mandrand-Berthelot Marie-Andree¹,Giordano Gerard²,Wu Long-Fei²

Affiliation:

1. Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, CNRS UMR5577, Institut National des Sciences Appliquées, 69621 Villeurbanne Cedex,1 and

2. Laboratoire de Chimie Bactérienne, CNRS UPR9043, Institut de Biologie Structurale et Microbiologie, 13402 Marseille Cedex 20,2 France

Abstract

ABSTRACT Expression of the nickel-specific transport system encoded by the Escherichia coli nikABCDE operon is repressed by a high concentration of nickel. By using random transposon Tn 10 insertion, we isolated mutants in which expression of the nik operon became constitutive with respect to nickel. We have identified the corresponding nikR gene which encodes a nickel-responsive regulator. Expression of nikR was partially controlled by Fnr through transcription from the nikA promoter region. In addition, a specific transcription start site for the constitutive expression of nikR was found 51 bp upstream of the nikR gene.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.181.2.670-674.1999

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