Affiliation:
1. Institut für Mikrobiologie, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle, Germany
Abstract
ABSTRACT
The Czc system of
Ralstonia
sp. strain CH34 mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB
2
A cation-proton antiporter. The CzcD protein is involved in the regulation of the Czc system. It is a membrane-bound protein with at least four transmembrane α-helices and is a member of a subfamily of the cation diffusion facilitator (CDF) protein family, which occurs in all three domains of life. The deletion of
czcD
in a
Ralstonia
sp. led to partially constitutive expression of the Czc system due to an increased transcription of the structural
czcCBA
genes, both in the absence and presence of inducers. The
czcD
deletion could be fully complemented in
trans
by CzcD and two other CDF proteins from
Saccharomyces cerevisiae
, ZRC1p and COT1p. All three proteins mediated a small but significant resistance to cobalt, zinc, and cadmium in
Ralstonia
, and this resistance was based on a reduced accumulation of the cations. Thus, CzcD appeared to repress the Czc system by an export of the inducing cations.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
187 articles.
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