Affiliation:
1. Division of Bacteria and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
2. Conselho Nacional de Desenvolvimento Científico e Técnologico
3. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil
Abstract
ABSTRACT
As a reference laboratory, the
Streptococcus
Laboratory at the Centers for Disease Control and Prevention (CDC) is frequently asked to confirm the identity of unusual or difficult-to-identify catalase-negative, gram-positive cocci. In order to accomplish the precise identification of these microorganisms, we have systematically applied analysis of whole-cell protein profiles (WCPP) and DNA-DNA reassociation experiments, in conjunction with conventional physiological tests. Using this approach, we recently focused on the characterization of three strains resembling the physiological groups I (strain SS-1730), II (strain SS-1729), and IV (strain SS-1728) of enterococcal species. Two strains were isolated from human blood, and one was isolated from human brain tissue. The results of physiological testing were not consistent enough to allow confident inclusion of the strains in any of the known enterococcal species. Resistance to vancomycin was detected in one of the strains (SS-1729). Analysis of WCPP showed unique profiles for each strain, which were not similar to the profiles of any previously described
Enterococcus
species. 16S ribosomal DNA (rDNA) sequencing results revealed three new taxa within the genus
Enterococcus
. The results of DNA-DNA relatedness experiments were consistent with the results of WCPP analysis and 16S rDNA sequencing, since the percentages of homology with all 25 known species of
Enterococcus
were lower than 70%. Overall, the results indicate that these three strains constitute three new species of
Enterococcus
identified from human clinical sources, including one that harbors the
vanA
gene. The isolates were provisionally designated
Enterococcus
sp. nov. CDC Proposed New Species of
Enterococcus
1 (CDC PNS-E1), type strain SS-1728
T
(= ATCC BAA-780
T
= CCUG 47860
T
);
Enterococcus
sp. nov. CDC PNS-E2, type strain SS-1729
T
(= ATCC BAA-781
T
= CCUG 47861
T
); and
Enterococcus
sp. nov. CDC PNS-E3, type strain SS-1730
T
(= ATCC BAA-782
T
= CCUG 47862
T
).
Publisher
American Society for Microbiology
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