Multiplex PCR Assay for Direct Identification of Group B Streptococcal Alpha-Protein-Like Protein Genes

Author:

Creti Roberta1,Fabretti Francesca1,Orefici Graziella1,von Hunolstein Christina1

Affiliation:

1. Laboratoriodi Batteriologia, Istituto Superiore di Sanità, 00161 Rome,Italy

Abstract

ABSTRACT We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference20 articles.

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2. Bolduc, G. R., M. J. Baron, C. Gravekamp, C. S. Lachenauer, and L. C. Madoff. 2002. The alpha C protein mediates internalization of group B streptococcus within human cervical epithelial cells. Cell Microbiol.4:751-758.

3. Borchardt, S. M., B. Foxman, D. Chaffin, C. Rubens, P. A. Tallman, S. D. Manning, C. J. Baker, and C. F. Marrs. 2003. Molecular serotyping of group B streptococci (GBS) by PCR and DNA dot blot hybridization.Ann. Epidemiol.13:581.

4. Creti R. J. L. Michel and G. Orefici. 2000. Genetic variability of the locus encoding alpha-C like proteins in Streptococcus agalactiae p. 397-399. In D. R. Martin and John Tagg (ed.) Streptococci and streptococcal diseases: entering the new millenium. Proceedings of the XIV Lancefield International Symposium on Streptococci and Streptococcal Diseases. Securacopy Porirua New Zealand.

5. Ferrieri, P. 1988. Surface-localized protein antigens of group B streptococci. Rev. Infect. Dis.10(Suppl. 2):S363-S366.

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