Affiliation:
1. Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Richmond, California 94804
Abstract
ABSTRACT
The identification of
Brucella
can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive
Brucella
isolates. The assay was designed in a multiplex format that will allow the rapid identification of
Brucella
spp.,
B. abortus
, and
B. melitensis
in a single test.
Publisher
American Society for Microbiology
Reference15 articles.
1. Baily, G. G., J. B. Krahn, B. S. Drasar, and N. G. Stoker. 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hygiene95:271-275.
2. Casanas, M. C., M. I. Queipo-Ortuno, A. Rodriguez-Torres, A. Orduna, J. D. Colmenero, and P. Morata. 2001. Specificity of a polymerase chain reaction assay of a target sequence on the 31-kilodalton Brucella antigen DNA used to diagnose human brucellosis. Eur. J. Clin. Microbiol. Infect. Dis.20:127-131.
3. Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: Evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene
4. Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR
5. Ficht, T. A., H. S. Husseinen, J. Derr, and S. W. Bearden. 1996. Species-specific sequences at the omp2 locus of Brucella type strains. Int. J. Syst. Bacteriol.46:329-331.
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