DifferentialIdentification of Mycobacterium tuberculosis Complexand Nontuberculous Mycobacteria by Duplex PCR Assay Using the RNAPolymerase Gene( rpoB )-Reference-Cited by-同舟云学术

DifferentialIdentification of Mycobacterium tuberculosis Complexand Nontuberculous Mycobacteria by Duplex PCR Assay Using the RNAPolymerase Gene( rpoB )

Published:2004-03 Issue:3 Volume:42 Page:1308-1312
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kim Bum-Joon¹,Hong Seong-Karp,Lee Keun-Hwa¹,Yun Yeo-Jun¹,Kim Eui-Chong²,Park Young-Gil³,Bai Gil-Han³,Kook Yoon-Hoh¹

Affiliation:

1. Departmentof Microbiology and Cancer Research Institute, Institute of EndemicDiseases, SNUMRC, Seoul National University College of Medicine, andClinical ResearchInstitute

2. Department ofClinical Pathology, Seoul National University College ofMedicine, Seoul110-799

3. The KoreanInstitute of Tuberculosis, Korean National TuberculosisAssociation, Seoul 137-140,Korea

Abstract

ABSTRACT A novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM), respectively, with two different sets of primers was used to differentially identify 44 reference strains and 379 clinical isolates of mycobacteria in a single-step assay. Showing 100% sensitivity and specificity, the duplex PCR method could clearly differentiate M . tuberculosis complex and NTM strains. In addition, restriction fragment length polymorphism analysis and direct sequencing of the amplicon of NTM could be used to supplement species identification.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.3.1308-1312.2004

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