Long-Term Preservation of Fungal Isolates in Commercially Prepared Cryogenic Microbank Vials

Author:

Espinel-Ingroff A.1,Montero D.2,Martin-Mazuelos E.2

Affiliation:

1. VCU Medical Center, Richmond, Virginia

2. Valme University Hospital, Seville, Spain

Abstract

ABSTRACT Since 1994, 6,198 yeasts and 391 molds belonging to 25 and 37 species, respectively, were stored in Microbank cryogenic vials at ≥−130°C in liquid nitrogen and at −70°C in a freezer. All of the isolates, with the exception of 45 yeasts and 15 dermatophytes, were recovered from both storage temperatures. Good reproducibility was demonstrated for amphotericin B, fluconazole, and voriconazole MICs determined for random isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

1. American Type Culture Collection. 1991. Preservation methods: freezing and freeze-drying 2nd ed. American Type Culture Collection Rockville Md.

2. Bakerspiegel, A. 1953. Soil as storage medium for fungi. Mycologia45:596-604.

3. Buell, C. B., and W. H. Weston. 1947. Application of the mineral oil conservation method for maintaining collection of fungal cultures. Am. J. Bot.34:555-561.

4. Carmichael, J. W. 1962. Viability of mold cultures stored at −20°C. Mycologia54:432-436.

5. Castellani, A. 1939. The viability of some pathogenic fungi in sterile distilled water. J. Trop. Med. Hyg.42:225-226.

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