Synthesis of d -Amino Acid-Containing Dipeptides Using the Adenylation Domains of Nonribosomal Peptide Synthetase

Abstract

Because almost all amino acids in nature are l -amino acids, the functioning of d -amino acids has received little attention. Thus, there is little information available on the activity of enzymes toward d -amino acids or synthetic methods for d -amino acid-containing dipeptides. Recently, d -amino acids and d -amino acid-containing peptides have attracted attention as novel functional compounds, and d -amino acid-activating enzymes and synthetic methods are required for the development of the d -amino acid-containing-peptide industry. This study provides additional knowledge regarding d -amino acid-activating enzymes and proposes a unique synthetic method for d -amino acid-containing peptides, including ld -, dl -, and dd -dipeptides.