Rapid Flow Cytometry Test for Identification of Different Carbapenemases in Enterobacteriaceae-Reference-Cited by-同舟云学术

Rapid Flow Cytometry Test for Identification of Different Carbapenemases in Enterobacteriaceae

Published:2016-06 Issue:6 Volume:60 Page:3824-3826
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Silva Ana P.¹²,Faria-Ramos Isabel¹²,Ricardo Elisabete¹²,Miranda Isabel M.¹²,Espinar Maria J.¹²³,Costa-de-Oliveira Sofia¹²^ORCID,Cantón Rafael⁴,Rodrigues Acácio G.¹²,Pina-Vaz Cidália¹²³

Affiliation:

1. Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal

2. Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal

3. Microbiology Department, Centro Hospitalar São João, Porto, Portugal

4. Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain

Abstract

ABSTRACT A flow cytometry test was developed to identify carbapenemase production by Enterobacteriaceae and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. It represents a convenient, fast, and reliable approach (100% sensitivity and 100% specificity) for the detection and characterization of different carbapenemases.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.02947-15

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