Author:
Wipf Juliette R. K.,Schwendener Sybille,Perreten Vincent
Abstract
ABSTRACTA novel erythromycin ribosome methylase gene,erm(44), that confers resistance to macrolide, lincosamide, and streptogramin B (MLSB) antibiotics was identified by whole-genome sequencing of the chromosome ofStaphylococcus xylosusisolated from bovine mastitis milk. Theerm(44) gene is preceded by a regulatory sequence that encodes two leader peptides responsible for the inducible expression of the methylase gene, as demonstrated by cloning inStaphylococcus aureus. Theerm(44) gene is located on a 53-kb putative prophage designated ΦJW4341-pro. The 56 predicted open reading frames of ΦJW4341-pro are structurally organized into the five functional modules found in members of the familySiphoviridae. ΦJW4341-pro is site-specifically integrated into theS. xylosuschromosome, where it is flanked by two perfect 19-bp direct repeats, and exhibits the ability to circularize. The presence oferm(44) in three additionalS. xylosusstrains suggests that this putative prophage has the potential to disseminate MLSBresistance.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
33 articles.
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